NF-kappa B Pathway in GBM tumors

  • February 11th, 2012
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Glioblastoma multiforme (GBM) is a malignant brain tumor characterized by uncontrolled growth, recruitment of new blood vessels, and resistance to cell death. Recent insights into the genetic and molecular changes that take place in GBM show that a protein called NF-kappa B is too abundant and too active inside the cell. NF-kappa B has several functions, including the prevention of cell death. The thinking is that having too much NF-kappa B is one reason GBM is resistant to cell death. This makes GBM more difficult to kill with chemotherapy and radiation. In fact, radiation and chemotherapy cause the cell to make even more NF-kappa B.

It has been shown that blocking NF-kappa B function decreases GBM growth. My goal is to study the other proteins in the NF-kappa B pathway and they way they are controlled. In this way, new strategies and treatments can be devised to interrupt this pathway and make GBM more susceptible to chemotherapy and radiation.

Investigators include: Ben Ayotte, MS candidate


Product Cat. No. Quantity Company Cost
RT2 Profiler™ PCR Array – NF-?B Signaling Targets PAHS-225A-12 12 arrays Qiagen $1757
RT2 SYBR Green/Fluorescin PCR Master Mix 330512 12 Qiagen $1136
RT2 First Strand Kit 330401 12 Qiagen $237
RNeasy Mini Kit 74104 12 Qiagen $57
RT2 PCR Array Loading Reservoir 338162 12 Qiagen $21.70
IFN-? (Recombinant Human) PHC4031 100 ?g Invitrogen $213.06
TNF-? (Recombinant Human) PHC3011 100 ?g Invitrogen $476.37
NF-?B p65 siRNA (Human) sc-29410 10 ?M Santa Cruz Biotechnology $258
NF-?B p65 Primer sc-29410-PR 10 ?M Santa Cruz Biotechnology $23
IRF-1 siRNA (Human) sc-35706 10 ?M Santa Cruz Biotechnology $258
IRF-1 Primer sc-35706-PR 10 ?M Santa Cruz Biotechnology $23
siRNA Reagent System sc-45064 Controls, reagents Santa Cruz Biotechnology $331
TOTAL: $4,991.13

Coming soon…

Coming soon…

Coming soon…